Formaldehyde interference with the o-toluidine procedure for glucose.
نویسندگان
چکیده
We recently noticed a significant error in the results for glucose determined by the o-toluidine method in qualitycontrol material prepared in our own laboratory. This interference was traced to one component of this control material, a uric acid stock solution containing formaldehyde. Uric acid has been reported to interfere (1, 2) and not interfere (3) in the o-toluidine procedure. The otoluidine method (4, 5 ) is based on the reaction between otoluidine and aldohexoses to form a complex mixture of colored products (6). Although this reaction is reportedly relatively specific for aldohexoses and pentoses (7-9), such statements refer to normal components of serum or urine (3, 8).
منابع مشابه
Automated determination of glucose in serum or plasma by a direct o-toluidine procedure.
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عنوان ژورنال:
- Clinical chemistry
دوره 20 10 شماره
صفحات -
تاریخ انتشار 1974